Journal: Translational Stroke Research

Article Title: Remote Ischemic Preconditioning Exerts Neuroprotective Effects Via the PGC-1α/FNDC5/BDNF Pathway in Focal Brain Ischemia of Rats

doi: 10.1007/s12975-026-01422-z

Figure Lengend Snippet: Effects of RIpreC on the expression of Bcl-2 ( A , B , F , G ), Bax ( A , C , F , H ), caspase-3 ( A , D , F , J ), and TUNEL-positive neurons ( A , E ) in the ischemic brain. Bcl-2 immunoreactivity in the perilesional cortex and its protein level were significantly increased in the RIpreC group compared the IR group. By contrast, Bax immunoreactivity and its protein level were significantly reduced in the RIpreC group compared with the IR group. The Bax/Bcl-2 ratios were also significantly reduced in the RIpreC group compared with the IR group ( I ). Subsequently, the number of TUNEL-positive neurons (arrow) and the ratios of caspase-3 positive to NeuN-positive neuron in the perilesional cortex were significantly decreased in the RIpreC group compared with the IR group ( E , K , L ). Mean ± standard error. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Scale bar = 50 μm ( A ) and 25 μm ( K ). ( n = 4–11 in each group)

Article Snippet: # p < 0.05 (Mann–Whitney U test), # p < 0.01 (Student’s t-test), Scale bar = 1 mm ( B ). ( n = 5–11 in each group) Co-localization of mouse anti-neuronal nuclei (NeuN) antibody (1:200; a marker of neurons; ab104224; Abcam plc, Cambridge, UK) with rabbit anti-caspase-3 antibody (1:200; 19677-1-AP; Proteintech, USA) immunoreactivities was examined using immunofluorescence staining.

Techniques: Expressing, TUNEL Assay

Journal: Translational Stroke Research

Article Title: Remote Ischemic Preconditioning Exerts Neuroprotective Effects Via the PGC-1α/FNDC5/BDNF Pathway in Focal Brain Ischemia of Rats

doi: 10.1007/s12975-026-01422-z

Figure Lengend Snippet: Effects of PGC-1αInhibition by SR-18,292. Inhibition of PGC-1α blunted the neuroprotective and neurorestorative effects of RIpreC ( A – F ). No changes in brain infarctions, neurological scores, and sensorimotor functions were observed in the RIpreC-treated SR-18,292 group compared with the IR group. Immunohistochemical analysis of the rectangular areas in A showed that immunoreactivities of PGC-1α, FNDC5, BDNF, and caspase-3 surrounding the lesion in the RIpreC-treated SR-18,292 group were similar to those in the IR group ( G – K ). Scale bar = 50 μm ( G ). ( n = 5–11 in each group)

Article Snippet: # p < 0.05 (Mann–Whitney U test), # p < 0.01 (Student’s t-test), Scale bar = 1 mm ( B ). ( n = 5–11 in each group) Co-localization of mouse anti-neuronal nuclei (NeuN) antibody (1:200; a marker of neurons; ab104224; Abcam plc, Cambridge, UK) with rabbit anti-caspase-3 antibody (1:200; 19677-1-AP; Proteintech, USA) immunoreactivities was examined using immunofluorescence staining.

Techniques: Inhibition, Immunohistochemical staining

Journal: Stem Cell Reports

Article Title: Histone methyltransferase Setd8 preserves chromatin accessibility to safeguard retinal progenitor cell identity during development

doi: 10.1016/j.stemcr.2025.102789

Figure Lengend Snippet: Setd8 deficiency reduces RPC proliferation and promotes apoptosis (A and D) Representative retinal section images of YFP (green) and EdU (magenta) staining at P0 (A) and P2 (D) from Ctrl (left) and Setd8 cKO (right) mice. Scale bars, 50 μm. (B and E) Quantification of the number of YFP+ (left graph) or EdU+ (right graph) cells at P0 (B) and P2 (E) ( n = 15 from 5 retinas). ∗∗ p < 0.01. (C and F) Proportion of YFP+ EdU+ (double-positive) cells among YFP+ cells at P0 (C) and P2 (F) ( n = 15 from 5 retinas). ∗∗ p < 0.01. (G) Representative retinal section images of γH2AX (magenta) staining at P0 (left) and P2 (right) from Ctrl (upper) and Setd8 cKO (lower) mice. Scale bars, 50 μm. (H) Quantification of the number of γH2AX + cells at P0 and P2 ( n = 15 from 5 retinas). (I) Representative retinal section images of YFP (green) and active caspase-3 (magenta) staining at P0 (left) and P2 (right) from Ctrl (upper) and Setd8 cKO (lower) mice. Scale bars, 50 μm. (J) Quantification of active caspase-3+ cells at P0 and P2 ( n = 15 from 5 retinas).

Article Snippet: The following primary antibodies were used: chicken anti-Nestin (1:500, NES, Aves Labs); goat anti-Sox2 (1:500, AF2018, R&D Systems); rabbit anti-active caspase 3 (1:500, AF-835, R&D Systems); chick anti-GFP (1:500, GFP-1010, Aves Labs); sheep anti-Chx10 (1:100, X1180P, Exalpha); rabbit anti-H4K20me1 (1:500, ab9051, Abcam); rabbit anti-S-opsin (1:500, AB5407, Millipore); rabbit anti-M-opsin (1:200, AB5405, Millipore); mouse anti-Brn3a (1:100, MAB1585, Millipore); mouse anti-Calbindin (1:500, CB300, Swant); goat anti-Otx2 (1:100, BAF1979, R&D Systems); mouse anti-Glutamine synthetase (1:500, 610517, BD Biosciences); rabbit anti-Pax6 (1:500, 901301, BioLegend); rabbit anti-γH2AX (1:500, ab2893, Abcam); and rat anti-BrdU (1:500, ab-6326, Abcam).

Techniques: Staining